Part:BBa_K2583000:Design

HRH4_CDS

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 706
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 856
  

Design Notes

The gaattc will be mutated to gagttc to avoid the restriction enzyme digestion site of EcoRI

Source

Homo sapiens:GRCh38.p12 (GCF_000001405.38)Chr 18 (NC_000018.10):24,457,882 - 24,490,113 Sequence found through GeneBank https://www.ncbi.nlm.nih.gov/gene/59340